Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Anti-Cancer Properties of Diethylether Extract of Wood from Sukun (Artocarpus altilis) in Human Breast Cancer (T47D) Cells

Enos Tangke Arung^1,2, Britanto Dani Wicaksono¹, Yohana Ayupriyanti Handoko¹, Irawan Wijaya Kusuma², Dina Yulia¹, Ferry Sandra¹

¹Stem Cell and Cancer Institute, Jalan Ahmad Yani no. 2 Pulomas, Jakarta, 13210; ²Wood Chemistry Laboratory, Forest Product Department, Forestry Faculty, Mulawarman University; Jalan KH. Dewantara, Kampus Gn. Kelua, Samarinda, East Kalimantan 75123, Indonesia.

For correspondence:- Ferry Sandra Email: fsandra@sci-indonesia.org Tel:+622147860173

Received: 6 February 2009 Accepted: 28 April 2009 Published: 14 August 2009

Citation: Arung ET, Wicaksono BD, Handoko YA, Kusuma IW, Yulia D, Sandra F. Anti-Cancer Properties of Diethylether Extract of Wood from Sukun (Artocarpus altilis) in Human Breast Cancer (T47D) Cells. Trop J Pharm Res 2009; 8(4):317-324 doi: 10.4314/tjpr.v8i4.5

© 2009 The authors.
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Abstract

Purpose: To evaluate the anti-cancer properties of the diethylether extract of Sukun (Artocarpus altilis) wood.

Methods: The extract was tested in human T47D breast cancer cells and examined for its effect on cell viability, nuclear morphology and sub-G1 formation. Cell viability was determined by microculture tetrazolium technique (MTT), nuclear morphology investigated using 4’-6-diamidino-2-phenylindole (DAPI) staining technique, and cell cycle progression monitored by sub-G1 apoptosis assay using flow cytometry.

Results: The results showed decreasing cell viability in a concentration-dependent manner. Altered cell morphology after treatment with the extract demonstrated that cells experienced apoptosis. Cell cycle analysis indicated that the number of cells in sub-G1 phase rose with increasing concentrations of the extract.

Conclusion: The data demonstrate that Sukun wood extract induced apoptosis and sub-G1 phase formation in breast cancer (T47D) cells, and therefore, has a potential as an anti-cancer agent.

Keywords: Artocarpus altili; Diethylether extract; T47D cells; Anti-cancer; Apoptosis; Sub-G1 formation